Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a
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چکیده
منابع مشابه
In vitro processing of dengue virus type 2 nonstructural proteins NS2A, NS2B, and NS3.
We have tested the hypothesis that the flavivirus nonstructural protein NS3 is a viral proteinase that generates the termini of several nonstructural proteins by using an efficient in vitro expression system and monospecific antisera directed against the nonstructural proteins NS2B and NS3. A series of cDNA constructs was transcribed by using T7 RNA polymerase, and the RNA was translated in ret...
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In a recent article (6), Wang et al. reported an extensive evaluation of NS1 antigen and compared it with real-time PCR, an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA), and a hemagglutination inhibition assay for diagnosis of dengue virus infection. The authors reported MAC-ELISA positivity for 46 (92%) out of 50 samples positive for dengue virus by real-time PCR (RT-PCR)...
متن کاملExpression of dengue virus structural proteins and nonstructural protein NS1 by a recombinant vaccinia virus.
A recombinant vaccinia virus containing cloned DNA sequences coding for the three structural proteins and nonstructural proteins NS1 and NS2a of dengue type 4 virus was constructed. Infection of CV-1 cells with this recombinant virus produced dengue virus structural proteins as well as the nonstructural protein NS1. These proteins were precipitated by specific antisera and exhibited the same mo...
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We report data from a prospective observational study performed in Martinique during a co-epidemic of dengue virus serotype 2 (DENV-2) and serotype 4 (DENV-4). Among 70 serum samples from patients with DENV-2 (n = 21) or DENV-4 (n = 49) infections, 47 (67.1%) were positive for dengue nonstructural protein 1 (NS1). Antigenemia correlated with plasma virus load and was independent of immune statu...
متن کاملImmunologic studies on the influenza A virus nonstructural protein NS1
We purified the major influenza virus nonstructural protein, designated NS1, from cytoplasmic inclusions that were solubilized and used to raise antisera in rabbits. One of the antisera was found to be specific for NS1 by complement fixation tests and analyses of immune precipitates. Antiserum to NS1 isolated from cells infected with A/WSN/33 virus specifically precipitated NS1 from extracts of...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1989
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.63.5.1852-1860.1989